Spectrophotometry is an analytical technique used to measure the intensity of light absorbed or transmitted by a sample as a function of wavelength. It plays a crucial role in various scientific fields, including chemistry, biology, and environmental science, by allowing researchers to study the concentration and properties of substances in solution. The process involves shining light through a sample and recording how much of it is absorbed at each specific wavelength. This information is used to determine the concentration of analytes, identify unknown substances based on their absorption spectra, or monitor chemical reactions. The Beer-Lambert Law is often employed in spectrophotometry to correlate the absorbance of light to the concentration of a solute in the solution.
