R is an open-source widely used programming languages in bioinformatics able to manipulate and analyze large datasets quickly and easily.

Background Dengue fever has rapidly manifested into a serious global health concern. The emergence of various viral serotypes has prompted the urgent need for innovative drug design techniques. Of the viral non-structural enzymes, the NS5 RNA-dependent RNA polymerase has been established as a promising target due to its lack of an enzymatic counterpart in mammalian cells and its conserved structure amongst all serotypes. The onus is now on scientists to probe further into understanding this enzyme and its mechanism of action. The field of bioinformatics has evolved greatly over recent decades, with updated drug design tools now being publically available. Methods In this study, bioinformatics tools were used to provide a comprehensive sequence and structural analysis of the two most prominent serotypes of Dengue RNA-dependent RNA polymerase. A list of popular flavivirus inhibitors were also chosen to dock to the active site of the enzyme. The best docked compound was then used as a template to generate a pharmacophore model that may assist in the design of target-specific Dengue virus inhibitors. Results Comparative sequence alignment exhibited similarity between all three domains of serotype 2 and 3.Sequence analysis revealed highly conserved regions at residues Meth530, Thr543 Asp597, Glu616, Arg659 and Pro671. Mapping of the active site demonstrated two highly conserved residues: Ser710 and Arg729. Of the active site interacting residues, Ser796 was common amongst all ten docked compounds, indicating its importance in the drug design process. Of the ten docked flavivirus inhibitors, NITD-203 showed the best binding affinity to the active site. Further pharmacophore modeling of NITD-203 depicted significant pharmacophoric elements that are necessary for stable binding to the active site. Discussion This study utilized publically available bioinformatics tools to provide a comprehensive framework on Dengue RNA-dependent RNA polymerase. Based on docking studies, a pharmacophore model was also designed to unveil the crucial pharmacophoric elements that are required when constructing an efficacious DENV inhibitor. We believe that this study will be a cornerstone in paving the road toward the design of target-specific inhibitors against DENV RdRp.

This cheatsheet simplifies the reverse_complement function, aiding bioinformatics students in understanding DNA strand complementation. Learn the basics of DNA nucleotide pairing and how to implement this in Python. For more tutorials and bioinformatics resources, feel free to reach out!

R Programming for Bioinformatics: Bioinformatics is a rapidly growing field that involves the use of computational tools to analyze large amounts of biological data.

This article presents the bioinformatics workflow of whole exome sequencing and related tools.

Bioinformatics has become an important field in Molecular Biology. Scientists utilize Bioinformatics tools for the analysis of genomic and transcriptomic data #Bioinformatics #Courses #Python #R #computationalbiology #biology

Importance of Bioinformatics is an integrative field for developing the technologies and tools of software to understand the biological data.

PRICES MAY VARY. Active, accessible, and assuming no prior knowledge: the ideal text for biologists encountering bioinformatics for the first time. A vast amount of biological information about a wide range of species has become available in recent years as technological advances have significantly reduced the time it takes to sequence a genome or determine a novel protein structure. This text describes how bioinformatics can be used as a powerful set of tools for retrieving and analysing this

After three back-to-back successful years, the Bioinformatics & Computational Biology Summer Program is back in 2023 as well. Like in previous years, it will be conducted online. This online program is built to give a broad overview of molecular biology, systems biology, and bioinformatics analysis through user-friendly software tools. Students will also learn how to apply

CanvasXpress (http://canvasxpress.org) was developed as the core visualization component for bioinformatics and systems biology analysis at Bristol-Myers Squibb and further enhanced by scientists around the world and served as a key visualization engine for many popular bioinformatics tools. It offers a rich set of interactive plots to display scientific and genomics data, such as oncoprint

Applications of deep-sequencing technologies in life science research and clinical diagnostics are rapidly expanding. Although fast data-processing algorithms exist, intuitive, portable data-evaluation solutions are still needed. Web tools have a history in bioinformatics of providing platform-independent, intuitive, barrier-free software solutions. Whereas in most scientific web tools a server performs intense calculations, the new HTML5 standard

A variety of extremely challenging biological sequence analyses were conducted on the XSEDE large shared memory resource Blacklight, using current bioinformatics tools and encompassing a wide range of scientific applications. These include genomic sequence assembly, very large metagenomic sequence assembly, transcriptome assembly, and sequencing error correction. The data sets used in these analyses included uncategorized

miRNAs play a key role in normal physiology and various diseases. miRNA profiling through next generation sequencing (miRNA-seq) has become the main platform for biological research and biomarker discovery. However, analyzing miRNA sequencing data is challenging as it needs significant amount of computational resources and bioinformatics expertise. Several web based analytical tools have been developed

Bioinformatic analysis can not only accelerate drug target identification and drug candidate screening and refinement, but also facilitate characterization o...

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Convenient, cost-effective, insightful and highly integrated platform of proteomics and bioinformatics services

Next-Generation Sequencing (NGS) is increasingly common and has applications in various fields such as clinical diagnosis, animal and plant breeding, and conservation of species. This incredible to...

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